Coldplay will open the show with a live performance from a pontoon on the River Thames. Dua Lipa, Arlo Parks and Headie One will play inside the venue.
About 4,000 people will watch the show, as part of the government's pilot scheme for live events.
Pop star Rag 'n' Bone man said it would be "really emotional" to play to fans for the first time in over a year.
"I'm not sure that I'm completely mentally prepared for it, but I'll give it my all," said the star, who'll be accompanied on stage by the Lewisham and Greenwich NHS Trust Choir.
US star Olivia Rodrigo, meanwhile, has spent the last week quarantining in a countryside hotel after flying into the UK to perform at the ceremony.
The singer, whose debut single Drivers License topped the charts for nine weeks earlier this year, says the Brits will mark the first time she's ever played her music for a live audience.
"Drivers License came out in quarantine, and I got to see the streaming numbers and the Tik Toks that people made - but it's a completely different experience to see people being affected by it in real life so I'm really, really excited."